Sensing of Human Blood Group by SPR Based Sensors

Sensing of Human Blood Group by SPR Based SensorsContents- universeBasic of Surface PlasmonP and S polarized crystalise beckonsProperties of Surface Plasmon Waves cross(prenominal) galvanising and Transverse Magnetic WavesDispersion Relation for Surface Plasmon convention list Internal ReflectionN-Layer regulate and Fresnels Relation impermanent Waves sonority ConditionMinimum of Reflectance at ResonanceDrude Model For free electron gasAngular Interrogation and Wavelength Interrogation exertion parameters for SPR set upd sensing elementsExperimentKretscheman and Otto ConfigurationCoating of surface layer by Vacuum Coating MachineBasic Of Surface Plasmons-The electron charges on a alloy bourn weed perform coherent fluctuations thusly collective charge density oscillations of electrons at metal-di galvanizing port wine argon cognise as plasma oscillations and the quantum of these oscillations atomic number 18 k straightwayn as come in plasmons. The come out of the clo e ven off plasmons are accompanied by a longitudinal (TM- or P-polarized) galvanizing field which decays exp one and only(a)ntially in metal as sanitary as in nonconductor modal(a). The electric field has its maximum at metal-dielectric user interface. The TM- polarization and exponential decay of electric field are found by solving the Maxwell equivalence. The charge motion in a locate plasmon always creates electro charismatic fields outside (as well as inside) the metal. The entireness excitation, including both the charge motion and associated electromagnetic field, is called either heighten plasmon polaritonat a planar interface. .Properties of Surface Plasmon Waves-Surface Plasmon jolts are the line up localized electromagnetic curls produced by the collective resonating oscillations of free electrons on the plasma near. The find Plasmon is a transverse roll up propagating along the plasma clear with the oscillating electric field vector normal to the appear. The deuce main properties of the arise Plasmon ripples are firstly they are of exponentially decaying nature, when propagate along the fold up of metal-dielecric interface it incurs decay both in metal as well as in dielectric and secondly it gets buckd only by P-polarized calamity luminosity-hearted kink.P-polarized and S-polarized light swans-When the electric field vector of chance light curl up is normal to the plane of incidence than light wave is called S-polarized light wave and when the electric field vector are replicate to plane of incidence than light wave is called P-polarized light wave.Transverse Electric and Transverse Magnetic WavesIf the incident, hereditary and reflected waves all thrust a bun in the oven a wave vector component along the z management, that is, they have an effective f number along z. The fields Ei,perp, Er,perp, and Et,perp are all perpendicular to the z direction. These waves are called transverse electric field (TE) waves. On the early(a) hand, waves with Ei,//, Er,//, and Et,// only have their magnetic field components perpendicular to the z direction and these are called transverse magnetic field (TM) waves.Dispersion Relation for Surface Plasmon-PrincipleTotal informal observation-Internal Reflectionis a phenomenon that happens when a propagatingwave strikes a spiritualist frontier at an locomote larger than a grouchy small weight downwith appreciate to thenormalto the surface. If the refractile officeis move on the other side of the demarcation line and the incident tip off is greater than the full of life angle, the wave female genital organ non pass through and is entirelyreflected. The comminuted angleis theangle of incidenceabove which the core privileged reflectance come ons. This is particularly common as anoptical phenomenon, where light waves are involved, tho it occurs with many types of waves, such aselectromagnetic wavesin general orsound waves. When a wave crosses a b oundary between materials with different kinds of deflective indices, the wave pull up stakes be partiallyrefractedat the boundary surface, and partially reflected. However, if the angle of incidence is greater (i.e. the direction of propagation or ray is closer to being parallel to the boundary) than the critical angle the angle of incidence at which light is refracted such that it travels along the boundary then the wave allow for not cross the boundary and instead be totally reflected indorse internally. This atomic number 50 only occur when the wave in a ordinary with a higher refractive index (n1) hits its surface thats in contact with a culture medium of lower refractive index (n2). For example, it will occur with light hittingairfrom glass, but not when hitting glass from air.Fresnels RelationWe will describe the incident, reflected and refracted waves by the exponential re endueation of a travelling wave, i.e.,Where r is theposition vector, the wavevectors ki, kr, kt describe individually the derictions of the incident, reflected and transmitted waves and Eio, Ero and Eto are the respective amplitudes. both phase changes such as phir and phit in the reflected and transmitted waves with respect to the phase of the incident wave are incorpo setd into the complex amplitudes Ero and Eto. Our objective is to find Ero and Eto with respect to Eio.Similar equations can be affirmd for the magnetic field components in the incident, reflected and transmitted waves but will be perpendicular to the corresponding electric fields. The electric and magnetic fields anywhere on the wave must be perpendicular to each other as a requirement of electromagnetic wave theary. This means that with E// in the EM wave we have a magnetic field Bperp associated with it such that Bperp= (n/c)E//. Simillarly, Eperp will have a magnetic field B// associated with it such that B//=(n/c)Eperp.There are both useful fundamental rules in electromagnetism that govern the beha viour of the electric and magnetic fields at a boundary between twain dielectric media which we can arbitrary label as 1 and 2. These rules are called boundary conditions. The first states that the electric field that is rambling to the boundary surface Etangential must be continuous across the boundary from medium 1 to 2 i.e., at the boundary y=0.Etangential(1)=Etangential(2)The second rule is that the tangential component of the magnetic field Btangential to boundary must be in any case continuous from medium 1 to 2 provided that the two media are non-magnetic ( relation back permeability r=1),Btangential(1)=Btangential(2)Using these boundary conditions for the fields at y=0, and the race between the electric and magnetic fields, we can find the reflected and transmitted waves in terms of the incident wave. The boundary conditions can only be live up to if the considerateness and incidence angles are fit, emailprotected, and the angles for the transmited and incident waves obey Snells emailprotectedemailprotectedApplying the boundary conditions to the EM wave going from medium 1 to medium 2, the amplitudes of the reflected and transmitted waves can be readily obtained in terms of n1, n2, and incidence angle i alone. These relationships are called Fresnels equations. If we define n=n2/n1, as the relative refractive index of medium 2 to that of 1, then the reflection and transmission system coefficients for Eperp areRperpAndTperpThese are corresponding coefficients for the E// fields with corresponding reflection and transmission coefficients r// and t//r//t//Further, the reflection and transmission coefficients are related byr// + nt// = 1 rperp +1 = tperpThe logical implication of these equations is that they allow the amplitudes and phases of the reflected and transmitted waves to be determined from the coefficients rperp, r//, t//, and tperp. passing waves-Evanescent waves are formed when sinusoidal waves are (internally) reflected off an interfa ce at an angle greater than the critical angle so that total internal reflection occurs. Evanescent means tending to vanish, which is appropriate because the potency of evanescent waves decays exponentially with distance from the interface at which they are formed. Any propagating wave is converted into an evanescent wave when hitting a classically forbidden region. In this case, at least one component of the wave vector kbecomes imaginary or complex and the wave experiences exponential damping when propagating in this region.In the case of an optical fictional character the cladding has less refractive index than the core of the fibre. When light experience total internal reflection at the core-cladding interface, about of the brawniness of the light waves in the core of the fibre penetrate into the cladding for a very short distance. The nix time period of this evanescent wave is parallel to the surface of the core and in the same direction as the main flow of energy within the core.Resonance Condition-The propagation unbroken (Ksp)Of the surface Plasmon wave propagating along the metal-dielectric interface is apt(p) by-Where m and s are the dielectric constants of metal and the dielectric medium respectively, is the absolute frequency of incident light, andc is the velocity of lightand from above equation it may be noted that the propagation constant of surface Plasmon wave depends on the dielectric constants of both the metal and the dielectric medium.The surface plasmons can be rided by light with same polarization state as that of surface Plasmons. The propagation constant (Ks) of the light wave with frequency w propagating through the dielectric medium is disposed by-Since ms0 (for dielectric), for a given frequency, the propagation constant of surface Plasmon (Ksp) is greater than that of light wave in dielectric medium (Ks). To excite surface plasmons, two propagation wave- vectors should be equal. Hence, the direct light cannot excite su rface plasmons at a metal-dielectric interface. To excite surface plasmons their momentum and therefrom the wave vector of the exciting light in dielectric medium should be increased. This can be do if instead of a direct light, evanescent wave is apply to excite the surface plasmons. To obtain the evanescent wave for the excitation of surface plasmons, a optical optical optical prism with high dielectric constant is used.When a light re is incident through one of the two sides of the prism at an angle greater than the critical angle at prism-air interface the total internal reflection of light beam takes place. In the condition of total internal reflection light beam does not return exactly from the interface. sort of it returns after penetrating in the lower refractive index medium (air in this case). The field in the lower refractive index medium is called evanescent field and the wave corresponding to this is called evanescent wave. The evanescent wave propagates along the prism-air interface and decays exponentially in the rarer medium (air). The propagation constant of the evanescent wave at prism-air interface is given by-Where,p, represents the dielectric constant of the material of the prism and is the angle of incidence of the beam.Increase in the dielectric constant of the prism increases the propagation constant of the evanescent wave and consequently this can be made equal to propagation constant of the surface Plasmon wave to propagation constant of the surface Plasmon wave to satisfy the surface Plasmon ring condition.When a p-polarized light beam is incident through the prism on the prism-metal layer interface at an angle equal to or greater than the critical angle, the evanescent wave is produced at the prism-metal interface. The excitation of surface plasmons occurs when the wave vector of evanescent wave exactly matches with that of the surface plasmons of akin frequency. This occurs at a particular angle of incidence res. Thus the resonance condition for surface Plasmon resonance is-The excitation of surface plasmons at metal-dielectric interface results in the transfer of energy from incident light to surface plasmons, which reduces the excitement of the reflected light. If the intensity of the reflected light is measured as a function of angle of incidence for a fixed values of frequency, metal layer weightiness and dielectric layer thickness then a sharp dusk is detect at resonance angle, res, due to an efficient transfer of energy to surface plasmons.Minimum of Reflectance at ResonanceThe excitation of surface plasmons at metal-dielectric interface results in the transfer of energy from incident photons to surface plasmons, which reduces the energy of the reflected light. If the normalized reflected intensity (R), which is basically the output signal, is measured as a function of incident angle by keeping other parameters and components (such as frequency, metal layer, and dielectric layer) unchange d, then a sharp dip is observed at resonance angle sp due to an efficient transfer of energy to surface plasmonsThe borderline of the normalized reflected intensity (R) can be quantitatively depict with the help of Fresnels equations for the three-layer system p/m/s. Here,p, stands for high refractive index material prismm, stands for metal film of thickness dm ands, stands for low index dielectric medium, eg air, water etc.The light wave is incident at an angle greater than the corresponding ATR angle. At this point, one outstanding fact is the energy conservation that requires R+A+T=1, that is, the sum of relative reflection, absorption, and transmission is unity. Since, T=0 at ATR, hence we are left with A+R=1 in the present case.The light wave having passed the glass prism (p), is reflected partially at prism interface. A part of the incident light wave energy traverses the metal film(of thickness dm) as an exponential decaying evanescent wave. At the metal-dielectric (m/s) i nterface, it induces the surface Plasmon excitation, which beam light back into the metal film. If the metal layer thickness (dm) is small, the back bemused field tends to increase. Since, this back scattered wave is out-of-phase with the incoming wave, the two interfere destructively and cause R to reduce. For minimum value of dm, they report each other and R becomes equal to zero. Thus, the absorption A becomes equal to 1, that is, whole radiation field is captured in the metal film. On the other hand, if the metal layer thickness is large enough, then the back scattered field disappears and R approaches to 1. It means that no absorption of incident light wave is taking place. Hence, one can dictate that the value of R depends on the combination of incident light frequency, angle of incidence, and the thickness of the metal layer.Performance parameters for SPR sensorsSensitivity and Detection accuracy are the two important parameters that are used to analyze the performance of SPR based sensors.Sensitivity- Suppose, we are sleuthing any medium of any particular refractive index, than we will get a minimum reflectance thin out at any particular value of res, but if change the medium than refractive index will change and we will get a minimum reflectance curve at any other value of res, hence we can say the minimum reflectance curve is arouseed from one wavelength value to another wavelength value. This shift in the minimum reflectance curve with the change in the refractive index (by changing the medium) is called sensitivity of the SPR sensor. Hence we can say, sensitivity is the rate of change of res value with the change in refractive index,S=If the shift is large, the sensitivity is largeDetection accuracy- How accurately and precisely our sensor can sense or detect this shift in minimum reflectance curve is called Detection Accuracy of the sensorDA=For the outgo performance of the sensor, both the parameters should be as high as potential to atta in a perfect sensing procedure.Otto ConfigurationThe general fancy behind this configuration was the coupling of surface Plasmon wave the evanescent wave, which is set up to ATR at the base of a coupling prism when a light beam is incident at an angle greater than the critical angle (ATR) at prism-air interface. The nature of evanescent wave is known to have the propagation constant along the interface and to decay exponentially in the dielectric medium adjacent to metal layer. Both of these characteristics of evanescent waveare akin to those of a surface Plasmon wave, therefore, there is a strong possibility of interaction between these waves. The x-component of the wave propagation constant of the evanescent wave at prism-air interface is given byKev=If a metal surface is now brought in contact of this decaying evanescent field in such a way that an air gap remains between the prism base and metal layer, then the evanescent field at prism-air interface can excite the surface pl asmons at the air-metal interface. However, this configuration is difficult to realize a great deal as the metal has to be brought within around 200 nm of the prism surface. This approach has been found to be very useful in studying single-crystal metal surfaces and adsorption on them.Kretschmann configurationAs a significant improvement to Otto configuration, Kretschmann and Reather realized that the metal layer could be used as the spacing layer, that is, evanescent wave generated at the prism-metal layer interface can excite surface plasmons at the metal-air interface so long as the metal layer thickness is not too large. They devised a new configuration. In this configuration as well, surface plasmons are worked up by an evanescent wave from a high refractive index glass prism at ATR condition. However, unlike Otto configuration, the base of the glass prism is coated with a thin metal film (typically around 50 nm thick) and is kept in direct contact with the dielectric medium of lower refractive index (such as air or some other dielectric sample).